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KMID : 0380020120270020086
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Korean Journal of Biotechnology and Bioengineering
2012 Volume.27 No. 2 p.86 ~ p.90
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Ethanol Production by Separate Hydrolysis and Fermentation and Simultaneous Saccharification and Fermentation Using Saccharina japonica
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Kim Min-Ji
Kim Sung-Koo
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Abstract
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Ethanol fermentations were carried out using simultaneous saccharification and fermentation (SSF) and separated hydrolysis and fermentation (SHF) processes with monosaccharides from seaweed, Saccharina japonica (sea tangle, Dasima) as the biomass. The pretreatment was carried out by thermal acid hydrolysis with H2SO4 or HCl. Optimal pretreatment condition was determined at 10% (w/v) seaweed slurry with 37.5 mM H2SO4 at 121℃ for 60 min. To increase the yield of saccharfication, isolated marine bacteria Bacillus sp. JS-1 was used and 48 g/L of reducing sugar were produced. Ethanol fermentation was performed using SSF and SHF process with Pachysolen tannophilus KCTC 7937. The ethanol concentration was 6.5 g/L by SSF and 6.0 g/L by SHF.
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KEYWORD
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Ethanol, Saccharina japonica, Pachysolen tannophilus, thermal acid hydorolysis
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